What kind of vials?

[newbiekat]

Got the results back!!

We tested 8 girls. One of them being Daisy, and if you have seen some of my previous threads, you know that's the one we have been struggling with for sometime now... Probably 6 mos or so, and still having issues.

Blood test:
We brought the buck home to run with the girls for a month. We didnt really have a way to separate Daisy, even though we really didnt want her bred in her condition. So, with that being said...
Of the four we hoped were bred, all four came back positive and Daisy did not take! Thank the LORD!

CAE:
All 8 came back NEGATIVE!!

Johnnes:
Out of the 8, Daisy turned out positive, and her daughter from this year (9 mos) also was positive. But I guess we did the ELISA test? When I talked to the vet, she said she has no doubt that Daisy has it, she shows the clinical signs of it. She did question her daughter having it due to the posible antibodies still in her system, so she suggested that we test with the AGID test for Daisy and her daughter. So we are going to go ahead and do that.

I'm still not quite clear on what Johnnes is. From what I understand, it is a disease that takes a few years to develop, and can remain "dormant" until then, showing no signs. Then for no apparent reason the goat can have a voracious appetite, yet still lose weight rapidly, and possibly have scours, but not always. And it can spread to other goats through the stool... Does that sound like I'm on the right track?

If those two come back positive on the AGID test, should I cull them? Well, we were planning on culling Daisy once we got her body codition back up (if we can), but what about her daughter?

 

[Southern by choice]

The Elisa test technically isn't for goats under 18 months.
Most of us do the test earlier.
You do have some options... the most accurate test is with a fecal. Takes 4 months.
If however, your vet really thinks she probably is a true positive which would be a safe bet given the daughter also tested positive then I would cull yesterday. Why get body condition up if you need to euthanize her?
The problem is it is on your land now. You will want to monitor your herd yearly.

http://www.johnes.org/general/faqs.html

Very glad you are CAE free!

Very sorry you are now dealing with Johnes.

I would contact the farm you got her from and give them the results, not accusing , just informing... they will probably want to test their herd.

 

[newbiekat]

Crud.

Well ok, then my question is this... Daisy is one of our original 3 girls. She is 4 1/2, she has two sisters from the same dam, but different sires. We also have another doe from this same farm, but different dam than the original 3 (it's DH's Grandfather's farm, CRUD!). Now, Daisy's daughter from last year tested negative, and the other three from this farm tested negative. It was just her daughter from this year... Any idea why that is?

Crud crud crud...

 

[Southern by choice]

Were the others given a blood test or did they do the fecal?

 

[newbiekat]

Blood test. ELISA.

 

[Southern by choice]

Looking at the site check out this- maybe you can call the lab and find out the number. Better yet your vet.
http://johnes.org/general/diagnosis.html

1. "ELISA.
   1. 2-3 milliliters of blood is collected from an adult animal. The fluid part of the sample (serum) is tested for anti-MAP antibody.
   2. The amount of antibody found (if any) is compared with positive and negative controls, and an interpretation is then assigned to the ELISA result. These numeric results (the actual amount of antibody) are useful: the higher the test result, the greater the certainty that the animal is infected and shedding MAP. "

Now look at what it says with the fecal test

. Organism-based tests. There are two types of these assays: (1) Culture, which isolates the living organism itself from manure, tissue or environmental samples and (2) PCR, which looks for the MAP genetic material from living or dead MAP.

1. Culture: A sample submitted for culture is monitored for seven weeks or longer because MAP is a very slow growing organism. If the sample is heavily contaminated with MAP, a positive result may be detected in a week or two, but it can take two months of incubation or more until the lab feels confident that no MAP organisms are present and can report a “culture negative” result.

If you can isolate maybe it is worth doing the second test depending on the numbers you /vet gets from the ELISA.